Full-genome sequence of human betacoronavirus 2c jordan-n3/2012 after serial passage in Mammalian cells

Kenneth G Frey; Cassie L Redden; Kimberly A Bishop-Lilly; Reed Johnson; Lisa E Hensley; Kanakatte Raviprakash; Thomas Luke; Tad Kochel; Vishwesh P Mokashi; Gabriel N Defang

doi:10.1128/genomeA.00324-14

Full-genome sequence of human betacoronavirus 2c jordan-n3/2012 after serial passage in Mammalian cells

Genome Announc. 2014 May 29;2(3):e00324-14. doi: 10.1128/genomeA.00324-14.

Authors

Kenneth G Frey¹, Cassie L Redden, Kimberly A Bishop-Lilly, Reed Johnson², Lisa E Hensley², Kanakatte Raviprakash³, Thomas Luke, Tad Kochel³, Vishwesh P Mokashi⁴, Gabriel N Defang⁵

Affiliations

¹ [email protected] [email protected].
² National Institutes of Health, National Institute of Allergy and Infectious Diseases, Integrated Research Facility, Fort Detrick, Maryland, USA.
³ Naval Medical Research Center, Viral and Rickettsial Diseases Department, Silver Spring, Maryland, USA.
⁴ Naval Medical Research Center, NMRC-Frederick, Fort Detrick, Maryland, USA.
⁵ Viral and Zoonotic Diseases Research Program, U.S. Naval Medical Research Unit 3, Abbassia, Cairo, Egypt [email protected] [email protected].

Abstract

Middle East respiratory syndrome coronavirus (MERS-CoV) is the etiologic agent of a highly lethal pneumonia. Here, we report the full-genome sequence of the Jordan-N3/2012 strain after serial passage in two distinct mammalian cell lines. The genome exhibits noteworthy stability, which may inform the development of vaccines and therapeutics used to treat infection with this virus.