Comparison of promoter DNA methylation and expression levels of genes encoding CCAAT/enhancer binding proteins in AML patients

Leuk Res. 2014 Jul;38(7):850-6. doi: 10.1016/j.leukres.2014.04.013. Epub 2014 May 9.

Abstract

CCAAT/enhancer binding proteins (CEBPs) are transcription factors regulating myeloid differentiation. Disturbances of their expression may contribute to leukemogenesis. In this study we compared promoter methylation and expression levels of selected CEBP genes in a group of 78 AML patients, normal bone marrow and hematopoietic precursor cells. CEBPA, CEBPD and CEBPE promoter methylation levels were elevated in 37%, 35.5% and 56.7% of patients. No CEBPZ(DDIT3) methylation was observed. An inverse relationship between CEBPA and CEBPD DNA methylation and expression levels was observed. AML cytogenetic risk groups and patients with particular translocation are characterized by distinct methylation/expression profile of CEBPs encoding genes.

Keywords: Acute myelogenous leukemia; CCAAT/enhancer binding protein; CEBPA; CEBPD; CEBPE; CEBPZ(DDIT3); DNA methylation; Gene expression.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • CCAAT-Enhancer-Binding Proteins / genetics*
  • DNA Methylation*
  • Female
  • Gene Expression Regulation, Leukemic*
  • Humans
  • Leukemia, Myeloid, Acute / genetics*
  • Male
  • Middle Aged
  • Promoter Regions, Genetic*
  • Translocation, Genetic

Substances

  • CCAAT-Enhancer-Binding Proteins