Six-month incidence and persistence of oral HPV infection in HIV-negative and HIV-infected men who have sex with men

PLoS One. 2014 Jun 4;9(6):e98955. doi: 10.1371/journal.pone.0098955. eCollection 2014.

Abstract

Objectives: Our aim was to assess incidence and persistence of oral HPV infection in HIV-negative and HIV-infected men who have sex with men (MSM).

Methods: MSM aged ≥18 years were included in Amsterdam (the Netherlands) in 2010-2011, and followed up 6 months later. Participants completed risk factor questionnaires. HPV DNA was analyzed in oral-rinse and gargle specimens using the SPF10-PCR DEIA/LiPA25 system (version 1). A subset of oral samples was subjected to SPF10 sequencing to identify additional HPV types. Multivariable logistic regression analyses using generalized estimating equations (GEE) were performed to assess determinants for oral high-risk HPV incidence and persistence.

Results: 689/795 participant MSM provided both baseline and 6-month data. Baseline prevalence of high-risk HPV was 9.4% in HIV-negative and 23.9% in HIV-infected MSM (P<0.001). 56/689 MSM acquired ≥1 high-risk HPV infection (6-month incidence 8.1%; 95%CI 6.2-10.4%); incidence was 4.1% in HIV-negative and 14.1% in HIV-infected MSM (P<0.001). HIV infection and recent use of cannabis were both independently associated with high-risk HPV incidence. Persistent high-risk HPV was observed in 48/130 (36.9%) infections.

Conclusion: Incidence of oral high-risk HPV infection in MSM is substantial, and is associated with HIV infection. Over a third of HPV infections persisted over a 6-month period.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • DNA, Viral / genetics
  • DNA, Viral / isolation & purification
  • Female
  • Follow-Up Studies
  • HIV Infections / complications*
  • HIV Infections / virology
  • HIV-1 / pathogenicity*
  • Homosexuality, Male*
  • Humans
  • Incidence
  • Male
  • Middle Aged
  • Mouth Diseases / epidemiology*
  • Mouth Diseases / virology
  • Netherlands / epidemiology
  • Papillomaviridae / genetics
  • Papillomaviridae / isolation & purification*
  • Papillomavirus Infections / epidemiology*
  • Papillomavirus Infections / virology
  • Polymerase Chain Reaction
  • Prevalence
  • Prognosis
  • Prospective Studies
  • Risk Factors
  • Surveys and Questionnaires
  • Time Factors
  • Young Adult

Substances

  • DNA, Viral

Grants and funding

This work was supported by Aids Fonds [grant number 2009029], and additional funding from the Public Health Service of Amsterdam and RIVM. Laboratory analysis of the 6-month oral samples was funded by Sanofi Pasteur MSD through an unrestricted grant. SPF10 sequencing of oral samples was funded by Stichting Pathologie Onderzoek en Ontwikkeling (SPOO). The Amsterdam Cohort Studies on HIV infection and AIDS, a collaboration between the Public Health Service of Amsterdam, the Academic Medical Center of the University of Amsterdam, Sanquin Blood Supply Foundation, and the University Medical Center Utrecht, are part of the Netherlands HIV Monitoring Foundation and financially supported by the Netherlands National Institute for Public Health and the Environment. DDL Diagnostic Laboratory provided support in the form of salaries for authors W. Quint, A. Molijn, and M. de Koning, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.