Effect of guanylate cyclase-C activity on energy and glucose homeostasis

Diabetes. 2014 Nov;63(11):3798-804. doi: 10.2337/db14-0160. Epub 2014 Jun 4.

Abstract

Uroguanylin is a gastrointestinal hormone primarily involved in fluid and electrolyte handling. It has recently been reported that prouroguanylin, secreted postprandially, is converted to uroguanylin in the brain and activates the receptor guanylate cyclase-C (GC-C) to reduce food intake and prevent obesity. We tested central nervous system administration of two GC-C agonists and found no significant reduction of food intake. We also carefully phenotyped mice lacking the GC-C receptor and found them to have normal body weight, adiposity, and glucose tolerance. Interestingly, uroguanylin knockout mice had a small but significant increase in body weight and adiposity that was accompanied by glucose intolerance. Our data indicate that the modest effects of uroguanylin on energy and glucose homeostasis are not mediated by central GC-C receptors.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cyclic GMP / metabolism
  • Eating / drug effects
  • Energy Metabolism / drug effects
  • Glucose / metabolism*
  • Guanylate Cyclase / metabolism*
  • Male
  • Mice
  • Natriuretic Peptides / pharmacology
  • Rats
  • Receptors, Enterotoxin
  • Receptors, Guanylate Cyclase-Coupled / metabolism
  • Receptors, Peptide / metabolism

Substances

  • Natriuretic Peptides
  • Receptors, Peptide
  • uroguanylin
  • Guanylate Cyclase
  • Gucy2c protein, mouse
  • Receptors, Enterotoxin
  • Receptors, Guanylate Cyclase-Coupled
  • Cyclic GMP
  • Glucose