Irradiation enhances expression of cxcr4 in murine glioma cells via HIF-1α-independent pathway

J Int Med Res. 2014 Aug;42(4):926-31. doi: 10.1177/0300060514533522. Epub 2014 Jun 4.

Abstract

Objective: To investigate levels of chemokine (C-X-C motif) receptor 4 (cxcr4) mRNA and protein in X-irradiated glioma cells.

Methods: Murine malignant glioma GL261 cells transfected with hypoxia-inducible factor (HIF)-1α miRNA or control miRNA were irradiated with X-radiation. Cxcr4 mRNA and protein were analysed using real-time reverse transcription-polymerase chain reaction and Western blot, respectively.

Results: Levels of cxcr4 protein in GL261 cells increased in a radiation dose-dependent manner 48 h after 0, 5, 10 and 15 Gy X-irradiation. Irradiation of both HIF-1α knockdown cells and control cells resulted in a significant increase in cxcr4 mRNA levels, compared with nonirradiated cells, at 24 h after 5 Gy X-irradiation.

Conclusion: Irradiation enhances expression of cxcr4 in glioma cells via a HIF-1α-independent pathway.

Keywords: CXCR4; Chemokine; glioma; neuropathology; radiotherapy.

MeSH terms

  • Animals
  • Blotting, Western
  • Cell Line, Tumor
  • Dose-Response Relationship, Radiation
  • Gene Knockdown Techniques
  • Glioma / radiotherapy*
  • Hypoxia-Inducible Factor 1, alpha Subunit / genetics*
  • Mice
  • MicroRNAs / genetics
  • RNA, Messenger / genetics*
  • Receptors, CXCR4 / genetics*
  • Receptors, CXCR4 / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • CXCR4 protein, mouse
  • Hif1a protein, mouse
  • Hypoxia-Inducible Factor 1, alpha Subunit
  • MicroRNAs
  • RNA, Messenger
  • Receptors, CXCR4