Abstract
Pancreatic cancer is characterized as a type of gastrointestinal tumor with a poor prognosis and high degree of malignancy. CIITA gene was found highly methylated in pancreatic carcinoma cell line PANC-1 and responsible for the low expression of MHC-II that may lead to immune evasion. Here, we tried to prepare pancreatic cancer vaccine with PANC-1 cells via epigenetic modification to enhance the MHC-II expression. Then the vaccine was injected into C57BL/6J mice and the effect was examined. Our study found that the vaccine could promote the proliferation of antigen-specific T cells, enhance the killing activity of cytotoxic lymphocytes (CTL), promote Th1-type cells mediated secretion of cytokines IFN-gamma and IL-2 while inhibiting Th2-type cells mediated secretion of IL-4, and inhibit the secretion of TGF-beta. Generally, the epigenetically modified vaccine could enhance the body's anti-tumor immune response, providing feasibility research on cancer vaccine for therapy of pancreatic cancer.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cancer Vaccines / genetics*
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Cancer Vaccines / immunology
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Cell Death / drug effects
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Cell Death / genetics
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Cell Line, Tumor
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Cell Proliferation / drug effects
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Cells, Cultured
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Cytokines / analysis
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Cytokines / metabolism
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DNA (Cytosine-5-)-Methyltransferases / antagonists & inhibitors
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DNA Methylation / drug effects
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DNA Methylation / genetics*
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Enzyme Inhibitors / pharmacology
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Epigenesis, Genetic / drug effects
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Epigenesis, Genetic / genetics*
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Genes, MHC Class II / drug effects
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Genes, MHC Class II / genetics
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Histone Deacetylase Inhibitors / pharmacology
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Humans
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Mice
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Mice, Inbred C57BL
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Nuclear Proteins / drug effects
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Nuclear Proteins / genetics
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Pancreatic Neoplasms / genetics
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Pancreatic Neoplasms / immunology*
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Spleen / cytology
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Th1 Cells / metabolism
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Th2 Cells / metabolism
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Trans-Activators / drug effects
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Trans-Activators / genetics
Substances
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Cancer Vaccines
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Cytokines
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Enzyme Inhibitors
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Histone Deacetylase Inhibitors
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MHC class II transactivator protein
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Nuclear Proteins
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Trans-Activators
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DNA (Cytosine-5-)-Methyltransferases