The distribution and common amino acid polymorphisms of human papillomavirus (HPV)-31 variants in 2700 women from Northern China

Mengfei Liu; Zhonghu He; Longfu Xi; Jingjing Li; Fangfang Liu; Ying Liu; Yaqi Pan; Tao Ning; Chuanhai Guo; Ruiping Xu; Lixin Zhang; Hong Cai; Yang Ke

doi:10.1371/journal.pone.0099141

The distribution and common amino acid polymorphisms of human papillomavirus (HPV)-31 variants in 2700 women from Northern China

PLoS One. 2014 Jun 5;9(6):e99141. doi: 10.1371/journal.pone.0099141. eCollection 2014.

Authors

Mengfei Liu¹, Zhonghu He¹, Longfu Xi², Jingjing Li¹, Fangfang Liu¹, Ying Liu¹, Yaqi Pan¹, Tao Ning¹, Chuanhai Guo¹, Ruiping Xu³, Lixin Zhang³, Hong Cai¹, Yang Ke¹

Affiliations

¹ Laboratory of Genetics, Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute, Beijing, P. R. China.
² Department of Pathology, School of Medicine, University of Washington, Seattle, Washington, United States of America.
³ Anyang Cancer Hospital, Anyang, Henan, P. R. China.

Abstract

To investigate the distribution of Human papillomavirus (HPV)-31 A, B and C variants as well as the common amino acid polymorphisms in Chinese women, all 14 HPV-31 positive cervical exfoliated cell specimens identified from a descriptive study including ∼2700 women from Northern China were analyzed. HPV-31 positive specimens were identified by Mass Spectrometry and the fragments of partial Long Control Region, E6 and E7 were amplified and directly sequenced or cloned into vector and then sequenced to confirm the variant information. HPV-31 prevalence in Northern Chinese female population was 0.52%. Six different sequences represented all 14 isolates, and these isolates were subsequently classified into variant lineage A (9), B (0) and C (5) by phylogenetic analysis. Five common amino acid polymorphism sites (2 in E6 and 3 in E7) and a novel non-synonymous mutation were detected in the current study. Our investigation suggested that HPV-31 was much less detected in Chinese women population than that in western countries. A and C variants were commonly detected while B variants were rarely detected in this population.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Aged
Cervix Uteri / metabolism
Cervix Uteri / virology*
China / epidemiology
Cohort Studies
Demography
Female
Gene Frequency
Genetic Variation*
Human papillomavirus 31 / classification
Human papillomavirus 31 / genetics*
Humans
Mass Spectrometry
Middle Aged
Mutation
Oncogene Proteins, Viral / genetics
Oncogene Proteins, Viral / metabolism
Papillomavirus E7 Proteins / genetics
Papillomavirus E7 Proteins / metabolism
Papillomavirus Infections / epidemiology
Papillomavirus Infections / virology*
Phylogeny

Substances

E6 protein, Human papillomavirus type 31
E7 protein, Human papillomavirus type 31
Oncogene Proteins, Viral
Papillomavirus E7 Proteins

Grants and funding

This work was supported by National Natural Science Foundation of China (grant number 81161120411, 30930102); "973" Project of National Ministry of Science and Technology Grant (grant number 2011CB504300, 2012CB910800); "863" Key Projects of National Ministry of Science and Technology Grant (grant number 2012AA02A209, 2012AA022502); Charity Project of National Ministry of Health (grant number 201202014, 200902002); the Specialized Research Fund for the Doctoral Program of Higher Education (20110001110037); and Natural Science Foundation of Beijing (grant number 7100001). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.