Emergence of VIM-2 and IMP-15 carbapenemases and inactivation of oprD gene in carbapenem-resistant Pseudomonas aeruginosa clinical isolates from Lebanon

Antimicrob Agents Chemother. 2014 Aug;58(8):4966-70. doi: 10.1128/AAC.02523-13. Epub 2014 Jun 9.

Abstract

We report here the emergence of VIM-2 and IMP-15 carbapenemases in a series of clinical isolates of carbapenem-resistant Pseudomonas aeruginosa in Lebanon. We also describe the disruption of the oprD gene by either mutations or insertion sequence (IS) elements ISPa1328 and ISPre2 isoform. Our study reemphasizes a rapid dissemination of the VIM-2 carbapenemase-encoding gene in clinical isolates of P. aeruginosa in the Mediterranean basin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacterial Typing Techniques
  • Carbapenems / pharmacology
  • DNA, Intergenic / chemistry
  • DNA, Intergenic / metabolism
  • Hospitals
  • Humans
  • Lebanon / epidemiology
  • Mutation
  • Phylogeny
  • Porins / deficiency
  • Porins / genetics*
  • Pseudomonas Infections / drug therapy
  • Pseudomonas Infections / epidemiology
  • Pseudomonas Infections / microbiology
  • Pseudomonas aeruginosa / classification*
  • Pseudomonas aeruginosa / drug effects
  • Pseudomonas aeruginosa / genetics*
  • Pseudomonas aeruginosa / isolation & purification
  • beta-Lactam Resistance / genetics*
  • beta-Lactamases / genetics*

Substances

  • Anti-Bacterial Agents
  • Carbapenems
  • DNA, Intergenic
  • Porins
  • OprD protein, Pseudomonas aeruginosa
  • beta-lactamase bla(vim-2)
  • beta-Lactamases
  • beta-lactamase IMP-15, Pseudomonas aeruginosa