In Saccharomyces cerevisiae fructose-1,6-bisphosphate contributes to the Crabtree effect through closure of the mitochondrial unspecific channel

Arch Biochem Biophys. 2014 Aug:555-556:66-70. doi: 10.1016/j.abb.2014.05.027. Epub 2014 Jun 9.

Abstract

In Saccharomyces cerevisiae addition of glucose inhibits oxygen consumption, i.e. S. cerevisiae is Crabtree-positive. During active glycolysis hexoses-phosphate accumulate, and probably interact with mitochondria. In an effort to understand the mechanism underlying the Crabtree effect, the effect of two glycolysis-derived hexoses-phosphate was tested on the S. cerevisiae mitochondrial unspecific channel (ScMUC). Glucose-6-phosphate (G6P) promoted partial opening of ScMUC, which led to proton leakage and uncoupling which in turn resulted in, accelerated oxygen consumption. In contrast, fructose-1,6-bisphosphate (F1,6BP) closed ScMUC and thus inhibited the rate of oxygen consumption. When added together, F1,6BP reverted the mild G6P-induced effects. F1,6BP is proposed to be an important modulator of ScMUC, whose closure contributes to the "Crabtree effect".

Keywords: Crabtree effect; Fructose-1,6-bisphosphate; Glucose-6-phosphate; Mitochondria; Permeability transition; Saccharomyces cerevisiae.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fructosediphosphates / metabolism*
  • Glucose / metabolism*
  • Glucose-6-Phosphate / metabolism
  • Glycolysis
  • Ion Channel Gating
  • Membrane Potential, Mitochondrial
  • Mitochondrial Swelling
  • Oxygen Consumption*
  • Potassium Channels / metabolism*
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / metabolism*

Substances

  • Fructosediphosphates
  • Potassium Channels
  • Saccharomyces cerevisiae Proteins
  • YMUC potassium channel protein, S cerevisiae
  • Glucose-6-Phosphate
  • Glucose
  • fructose-1,6-diphosphate