Comparison of the immunoperoxidase monolayer assay and three commercial ELISAs for detection of antibodies against porcine circovirus type 2

Vet J. 2014 Sep;201(3):429-32. doi: 10.1016/j.tvjl.2014.05.025. Epub 2014 May 22.

Abstract

The aim of this study was to compare and correlate antibody titres against porcine circovirus type 2 (PCV2) in porcine sera (n = 1270) obtained by immunoperoxidase monolayer assay (IPMA) with the results of three commercial ELISAs (designated E1, E2 and E3). The correlation between IPMA and ELISA results was excellent (r(2) ≥ 0.90). Compared to IPMA, E2 had the highest sensitivity (93.0%), followed by E3 (90.1%) and E1 (85.0%); the specificity was 100% for all tests. All three commercial ELISAs had predictive values similar to those of IPMA and could be used to monitor antibody responses against PCV2 infection and/or vaccination.

Keywords: ELISA; Immunoperoxidase monolayer assay; Porcine circovirus type 2; Serology.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / blood*
  • Circoviridae Infections / diagnosis
  • Circoviridae Infections / veterinary*
  • Circoviridae Infections / virology
  • Circovirus / immunology*
  • Enzyme-Linked Immunosorbent Assay / veterinary*
  • Female
  • Immunoenzyme Techniques / veterinary*
  • Swine
  • Swine Diseases / diagnosis*
  • Swine Diseases / virology

Substances

  • Antibodies, Viral