Characteristic mode of action of gangliosides in selective modulation of CD4 on human T lymphocytes

Biochem Biophys Res Commun. 1989 Feb 15;158(3):1050-9. doi: 10.1016/0006-291x(89)92828-3.

Abstract

We have explored the possible mechanisms for selective modulation by gangliosides of CD4 on human T lymphocytes and subsequent re-expression of CD4. Indirect immunofluorescence staining with anti-CD4 antibodies revealed newly internalized CD4 in ganglioside-treated cells after membrane permeabilization with 0.1% saponin. Cycloheximide and other metabolic inhibitors did not alter the modulation but inhibited significantly the re-expression of CD4. These results suggest both selective modulation of CD4 by a process of endocytosis and re-expression of CD4 through de novo protein synthesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Differentiation, T-Lymphocyte*
  • Blotting, Western
  • Cell Membrane / immunology
  • Cell Membrane Permeability / drug effects
  • Cholera Toxin
  • Dose-Response Relationship, Drug
  • Fluorescein-5-isothiocyanate
  • Fluoresceins
  • Fluorescent Antibody Technique
  • Fluorescent Dyes
  • G(M1) Ganglioside / pharmacology
  • Gangliosides / pharmacology*
  • Humans
  • Leukemia, T-Cell
  • Saponins / pharmacology
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / immunology*
  • Thiocyanates
  • Tumor Cells, Cultured

Substances

  • Antigens, Differentiation, T-Lymphocyte
  • Fluoresceins
  • Fluorescent Dyes
  • Gangliosides
  • Saponins
  • Thiocyanates
  • G(M1) Ganglioside
  • Cholera Toxin
  • Fluorescein-5-isothiocyanate