Rhodium(III)-catalyzed regioselective C2-amidation of indoles with N-(2,4,6-trichlorobenzoyloxy)amides and its synthetic application to the development of a novel potential PPARγ modulator

Org Biomol Chem. 2014 Sep 21;12(35):6831-6. doi: 10.1039/c4ob00637b.

Abstract

A new and efficient method for the direct regioselective C2-amidation of various functionalized indoles with several N-(2,4,6-trichlorobenzoyloxy)amides via Rh(iii)-catalyzed C-H activation/N-O cleavage/C-N formation using the pyrimidyl group as a readily installable and removable directing group has been developed. With this method, a variety of valuable 2-amido indoles can be easily prepared under mild conditions with broad functional group tolerance and excellent region-/site-specificities. Application of this strategy to the synthesis of target compound as a novel PPARγ modulator was also demonstrated. The results from biological evaluation showed that compound had a partial PPARγ agonistic activity and a strong PPARγ binding affinity with an IC50 value of 120.0 nM, along with a less pronounced adipocyte differentiation ability compared to the currently marketed anti-diabetic drug rosiglitazone, suggesting that further development of such a compound might be of great interest.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes / cytology
  • Adipose Tissue / metabolism
  • Amides / chemistry*
  • Animals
  • COS Cells
  • Carbon / chemistry
  • Catalysis
  • Cell Differentiation
  • Chemistry, Organic / methods
  • Chlorocebus aethiops
  • Drug Design
  • Hydrogen / chemistry
  • Indoles / chemistry*
  • Inhibitory Concentration 50
  • Molecular Structure
  • Nitrogen / chemistry
  • Oxygen / chemistry
  • PPAR gamma / chemistry*
  • Protein Binding
  • Rhodium / chemistry*
  • Rosiglitazone
  • Thiazolidinediones / chemistry

Substances

  • Amides
  • Indoles
  • PPAR gamma
  • Thiazolidinediones
  • Rosiglitazone
  • Carbon
  • Hydrogen
  • Rhodium
  • Nitrogen
  • Oxygen