Abstract
Brain-derived neurotrophic factor (BDNF) exerts therapeutic effects following intracerebral hemorrhage (ICH). However, it is difficult to maintain sufficient concentrations in the hemorrhage hemisphere. We demonstrated previously that BDNF fused to a collagen-binding domain (CBD) could bind to collagen in the ventricular ependyma and stimulate cell proliferation in the subventricular zone (SVZ). In this study, we verified the therapeutic effects of CBD-BDNF in the rat ICH model induced by bacterial collagenase by injecting CBD-BDNF into the lateral ventricle of ICH rats. The results demonstrated that CBD-BDNF was retained at high levels in the hemorrhage hemisphere, where it promoted neural regeneration and angiogenesis, reduced tissue loss, and improved functional recovery.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Behavior, Animal
-
Brain / blood supply
-
Brain / metabolism
-
Brain / pathology
-
Brain Edema / complications
-
Brain Edema / drug therapy
-
Brain Edema / pathology
-
Brain Edema / physiopathology
-
Brain-Derived Neurotrophic Factor / pharmacology
-
Brain-Derived Neurotrophic Factor / therapeutic use*
-
Cell Differentiation / drug effects
-
Cell Proliferation / drug effects
-
Cerebral Hemorrhage / complications
-
Cerebral Hemorrhage / drug therapy*
-
Cerebral Hemorrhage / pathology
-
Cerebral Hemorrhage / physiopathology*
-
Collagen / metabolism*
-
Disease Models, Animal
-
Glucose / metabolism
-
Hematoma / complications
-
Hematoma / drug therapy
-
Hematoma / pathology
-
Hematoma / physiopathology
-
Hemodynamics / drug effects
-
Immunohistochemistry
-
Magnetic Resonance Imaging
-
Male
-
Neovascularization, Physiologic / drug effects
-
Nerve Regeneration* / drug effects
-
Neurogenesis / drug effects
-
Positron-Emission Tomography
-
Protein Binding / drug effects
-
Rats, Sprague-Dawley
-
Recombinant Fusion Proteins / pharmacology
-
Recombinant Fusion Proteins / therapeutic use*
-
Recovery of Function* / drug effects
-
Tomography, Emission-Computed, Single-Photon
Substances
-
Brain-Derived Neurotrophic Factor
-
Recombinant Fusion Proteins
-
Collagen
-
Glucose