Expression pattern of heme oxygenase 1 gene and hypoxic adaptation in chicken embryos

Comp Biochem Physiol B Biochem Mol Biol. 2014 Aug:174:23-8. doi: 10.1016/j.cbpb.2014.05.005. Epub 2014 Jun 16.

Abstract

Heme oxygenase 1 (HO-1), a rate-limiting enzyme of heme catabolism, has a crucial role of cytoprotective functions under hypoxia. The objective of the present study was to investigate potential differences in protective effect of HO-1 gene on chicken (Gallus gallus) embryo lung during late incubation. At embryonic day (D) D16, D18, D19, and D20 of incubation, the expression of HO-1 in the lungs of chicken embryos (Tibet and Shouguang chickens) incubated in normoxic (21% O2) and hypoxic (13% O2) conditions was measured. SNPs were screened within 5'-flanking region and coding regions with PCR-sequencing and the genotype of the SNPs was determined with PCR-RFLP in Tibet, Chahua and Shouguang chicken populations. In conclusion, the Tibet chicken had higher HO-1 expression on D19 under hypoxic incubation and had two SNPs with different frequency distributions from other chicken breeds, which might be a way that the Tibet chicken had hereditary adaptation to hypoxia during embryonic development.

Keywords: Expression pattern; HO-1; Hypoxic adaptation; Tibet chicken.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptation, Biological
  • Animals
  • Chick Embryo
  • Genotype
  • Heme Oxygenase-1 / genetics
  • Heme Oxygenase-1 / metabolism*
  • Hypoxia / embryology
  • Hypoxia / metabolism*
  • Lung / embryology
  • Lung / metabolism
  • Polymorphism, Single Nucleotide

Substances

  • Heme Oxygenase-1