Expression of serum amyloid A4 in human trophoblast-like choriocarcinoma cell lines and human first trimester/term trophoblast cells

Placenta. 2014 Aug;35(8):661-4. doi: 10.1016/j.placenta.2014.05.012. Epub 2014 Jun 7.

Abstract

Trophoblast invasion into uterine tissues represents a hallmark of first trimester placental development. As expression of serum amyloid A4 (SAA4) occurs in tumorigenic and invasive tissues we here investigated whether SAA4 is present in trophoblast-like human AC1-M59/Jeg-3 cells and trophoblast preparations of human first trimester and term placenta. SAA4 mRNA was expressed in non-stimulated and cytokine-treated AC1-M59/Jeg-3 cells. In purified trophoblast cells SAA4 mRNA expression was upregulated at weeks 10 and 12 of pregnancy. Western-blot and immunohistochemical staining of first trimester placental tissue revealed pronounced SAA4 expression in invasive trophoblast cells indicating a potential role of SAA4 during invasion.

Keywords: Cytokine; Invasion; Outgrowing extravillous trophoblast; Placenta; SAA4.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Female
  • Humans
  • Pregnancy
  • Pregnancy Trimester, First
  • Pregnancy Trimester, Third
  • Serum Amyloid A Protein / metabolism*
  • Trophoblasts / metabolism*

Substances

  • SAA4 protein, human
  • Serum Amyloid A Protein