Isolation of West Nile virus from urine samples of patients with acute infection

Luisa Barzon; Monia Pacenti; Elisa Franchin; Laura Squarzon; Alessandro Sinigaglia; Sebastian Ulbert; Riccardo Cusinato; Giorgio Palù

doi:10.1128/JCM.01328-14

Isolation of West Nile virus from urine samples of patients with acute infection

J Clin Microbiol. 2014 Sep;52(9):3411-3. doi: 10.1128/JCM.01328-14. Epub 2014 Jun 20.

Authors

Luisa Barzon¹, Monia Pacenti², Elisa Franchin³, Laura Squarzon³, Alessandro Sinigaglia², Sebastian Ulbert⁴, Riccardo Cusinato², Giorgio Palù³

Affiliations

¹ Department of Molecular Medicine, University of Padua, Padua, Italy Microbiology and Virology Unit, Padua University Hospital, Padua, Italy [email protected].
² Microbiology and Virology Unit, Padua University Hospital, Padua, Italy.
³ Department of Molecular Medicine, University of Padua, Padua, Italy Microbiology and Virology Unit, Padua University Hospital, Padua, Italy.
⁴ Department of Immunology, Fraunhofer Institute for Cell Therapy and Immunology, Leipzig, Germany.

Abstract

This study demonstrated that West Nile virus (WNV) excreted in the urine of patients with acute infection can be isolated in cell cultures. In addition, the protocols for WNV isolation from urine samples were standardized, and factors that may affect the efficiency of WNV isolation were identified.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Diagnostic Tests, Routine / methods
Diagnostic Tests, Routine / standards
Humans
Urine / virology*
Virus Cultivation / methods
Virus Cultivation / standards
West Nile Fever / virology*
West Nile virus / isolation & purification*