Differences in 23 S rRNA-protein neighbourhood in Escherichia coli 70 S ribosomes and 70 S initiation complex. Probing by bifunctional Pt(II)-containing reagent

FEBS Lett. 1989 Mar 27;246(1-2):197-201. doi: 10.1016/0014-5793(89)80282-0.

Abstract

rRNA-protein cross-links in free E. coli 35S-labeled 70 S ribosomes and in the initiation complex 35S-labeled 70 S ribosome.AUGU6.fMet-tRNA(fMet) were studied with the aid of a new type of binuclear Pt(II) compound - dichlorotetra-ammine(1,6-hexamethylenediaminediplatinum++ +) dichloride. The use of this reagent allowed us to reveal differences in the rRNA-protein neighbourhood in free 70 S ribosomes and in the initiation complex. Proteins L3, L6, L23 and L25 were shown to cross-link to 23 S rRNA only in the initiation complex, whereas proteins L1, L13, L14, L16, L17, L18, L22, L28 and S1 did so in both free ribosomes and the complex. 16 S rRNA was found to be cross-linked preferentially to a single protein, S1, in both states of the ribosomes.

Publication types

  • Comparative Study

MeSH terms

  • Centrifugation, Density Gradient
  • Cross-Linking Reagents
  • Electrophoresis, Gel, Two-Dimensional
  • Escherichia coli / metabolism*
  • Organoplatinum Compounds / pharmacology*
  • RNA, Ribosomal / metabolism*
  • RNA, Ribosomal, 23S / metabolism*
  • RNA, Transfer, Amino Acyl / metabolism
  • RNA, Transfer, Met*
  • RNA, Transfer, Phe / metabolism
  • Ribosomal Proteins / metabolism*
  • Ribosomes / metabolism*
  • Thiourea / pharmacology

Substances

  • Cross-Linking Reagents
  • Organoplatinum Compounds
  • RNA, Ribosomal
  • RNA, Ribosomal, 23S
  • RNA, Transfer, Amino Acyl
  • RNA, Transfer, Met
  • RNA, Transfer, Phe
  • Ribosomal Proteins
  • tRNA, formylmethionine-
  • dichlorotetraamine(1,6-hexamethylenediamine)diplatinum(II)
  • Thiourea