Response of murine IL3-sensitive cell lines to cytokines of human and murine origin

Lymphokine Res. 1989 Spring;8(1):79-84.

Abstract

We analyzed the proliferative response of DA1, DA1-a, NFS 60, 32DC1, Ea 3-17 and FDCP2 murine interleukin 3 (mIL3)-sensitive cell lines to human recombinant IL1 alpha, IL1 beta, IL2, IL4, IL5, IL6, granulocyte-CSF (G-CSF), macrophage-CSF (M-CSF), granulocyte-macrophage-CSF (GM-CSF), interferon gamma (IFN gamma), tumor necrosis factor (TNF) alpha, gibbon IL3, purified HILDA and mouse recombinant IL3, IL4, GM-CSF. All cell lines responded to mIL3 and IL4. Various response patterns were observed with human IL2, G-CSF and murine GM-CSF. Human IL4 and GM-CSF were absolutely inefficient in triggering proliferation of lines sensitive to their murine counterparts, confirming the species specificity of these two cytokines. Among the cell lines responding to G-CSF, some variations in sensitivities were observed. Thus the 32DC1 cell line needed 10 to 30 times more G-CSF to reach the same level of proliferation as the NFS60 cell line. DA1-a was the only factor-dependent cell line responding to purified HILDA, and IL6 was found to trigger proliferation of the NFS60 cell line, with a half-maximum effect observed for 0.1 pM of hormone.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Biological Factors / pharmacology*
  • Cell Division / drug effects
  • Cell Line
  • Cytokines
  • Growth Inhibitors*
  • Humans
  • Interleukin-3 / pharmacology*
  • Interleukin-6*
  • Leukemia Inhibitory Factor
  • Lymphokines / analysis
  • Mice

Substances

  • Biological Factors
  • Cytokines
  • Growth Inhibitors
  • Interleukin-3
  • Interleukin-6
  • LIF protein, human
  • Leukemia Inhibitory Factor
  • Lif protein, mouse
  • Lymphokines