Genome scale cytometry: High content analysis for high throughput RNAi phenotype profiling

David M Evans; David O Azorsa; Spyro Mousses

doi:10.1016/j.ddtec.2005.05.012

Genome scale cytometry: High content analysis for high throughput RNAi phenotype profiling

Drug Discov Today Technol. 2005 Summer;2(2):141-7. doi: 10.1016/j.ddtec.2005.05.012.

Authors

David M Evans¹, David O Azorsa², Spyro Mousses²

Affiliations

¹ Cancer Drug Development Laboratory, Translational Genomics Research Institute, 20, Firstfield Rd, Gaithersburg, MD 20878, USA. Electronic address:[email protected].
² Cancer Drug Development Laboratory, Translational Genomics Research Institute, 20, Firstfield Rd, Gaithersburg, MD 20878, USA.

PMID: 24981841
DOI: 10.1016/j.ddtec.2005.05.012

Abstract

The combination of RNAi-mediated knockdown of gene expression and high content screening (HCS) allows the determination of the contribution of single genes to a variety of cellular effects varying between growth and survival to subtle alterations in cellular morphology and phenotype. This review examines the current status of research in combining these tools.: