Next-generation sequencing fragment library construction

Jessica Podnar; Heather Deiderick; Scott Hunicke-Smith

doi:10.1002/0471142727.mb0717s107

Next-generation sequencing fragment library construction

Curr Protoc Mol Biol. 2014 Jul 1:107:7.17.1-7.17.16. doi: 10.1002/0471142727.mb0717s107.

Authors

Jessica Podnar¹, Heather Deiderick¹, Scott Hunicke-Smith¹

Affiliation

¹ Genomic Sequencing and Analysis Facility, University of Texas at Austin, Austin, Texas.

PMID: 24984855
DOI: 10.1002/0471142727.mb0717s107

Abstract

All current next-generation sequencing (NGS) platforms and applications require the sequencing library to have specific characteristics: in particular, size, size distribution, and 5' and 3' flanking sequences. This unit presents a robust protocol for converting a wide variety of input DNA samples into appropriate NGS libraries and discusses important considerations in experimental design, failure modes, and typical results.

Keywords: DNA; NGS; amplicon; fragment; libraries; library; sequencing; shotgun.

Publication types

Review

MeSH terms

Animals
Gene Library*
High-Throughput Nucleotide Sequencing / methods*
Humans
Sequence Analysis, DNA / methods*