Lactones from Ficus auriculata and their effects on the proliferation function of primary mouse osteoblasts in vitro

Bioorg Med Chem Lett. 2014 Aug 15;24(16):3952-5. doi: 10.1016/j.bmcl.2014.06.035. Epub 2014 Jun 21.

Abstract

Bioassay-guided fractionation of the petroleum ether, chloroform and EtOAc extracts of the stems of Ficus auriculata led to the isolation of five new 12-membered lactones (3R,4R)-4-hydroxy-de-O-methyllasiodiplodin (1), 6-oxolasiodiplodin (2) and ficusines A-C (3-5), together with three known related analogues (6-8). The structures of the new compounds were elucidated by comprehensive spectroscopic data. The absolute configurations of 3 and 8 were established by single crystal X-ray diffraction analysis. Compounds 3-5 represent the first 12-membered lactones with a quinone ring unit. Compounds 6 and 7 exhibited significant proliferation function of primary osteoblasts (OBs) in vitro. Especially, the promotion rate of 6 reached 151.55±1.34% (P<0.001) at the concentration of 100 μM.

Keywords: Chemical constituents; Ficus auriculata; Lactones; Primary osteoblasts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Proliferation / drug effects
  • Dose-Response Relationship, Drug
  • Ficus / chemistry*
  • Lactones / chemistry
  • Lactones / isolation & purification
  • Lactones / pharmacology*
  • Mice
  • Molecular Structure
  • Osteoblasts / cytology*
  • Osteoblasts / drug effects*
  • Structure-Activity Relationship

Substances

  • Lactones