Comparison of two real-time RT-PCR assays for differentiation of C-strain vaccinated from classical swine fever infected pigs and wild boars

Res Vet Sci. 2014 Oct;97(2):455-7. doi: 10.1016/j.rvsc.2014.06.010. Epub 2014 Jul 1.

Abstract

Classical swine fever is one of the most important infectious diseases for the pig industry worldwide due to its economic impact. Vaccination is an effective means to control disease, however within the EU its regular use is banned owing to the inability to differentiate infected and vaccinated animals, the so called DIVA principle. This inability complicates monitoring of disease and stops international trade thereby limiting use of the vaccine in many regions. The C-strain vaccine is safe to use and gives good protection. It is licensed for emergency vaccination in the EU in event of an outbreak. Two genetic assays that can distinguish between wild type virus and C-strain vaccines have recently been developed. Here the results from a comparison of these two real-time RT-PCR assays in an interlaboratory exercise are presented. Both assays showed similar performance.

Keywords: C-strain; CSFV; Classical swine fever; DIVA; PCR; Vaccine.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / genetics
  • Antibodies, Viral / immunology
  • Classical Swine Fever / diagnosis*
  • Classical Swine Fever / immunology*
  • Classical Swine Fever Virus / genetics*
  • Classical Swine Fever Virus / immunology
  • DNA, Viral / genetics
  • Diagnosis, Differential
  • Diagnostic Tests, Routine / veterinary
  • European Union
  • Genotype
  • Real-Time Polymerase Chain Reaction / methods*
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Sus scrofa / virology*
  • Swine / virology*
  • Vaccination / veterinary
  • Viral Vaccines / classification
  • Viral Vaccines / genetics*
  • Viral Vaccines / immunology

Substances

  • Antibodies, Viral
  • DNA, Viral
  • Viral Vaccines