From molecular evolution to biobricks and synthetic modules: a lesson by the bacterial flagellum

Biotechnol Genet Eng Rev. 2014 Oct;30(1-2):49-64. doi: 10.1080/02648725.2014.921500.

Abstract

The bacterial flagellum is a motility structure and represents one of the most sophisticated nanomachines in the biosphere. Here, we review the current knowledge on the flagellum, its architecture with respect to differences between Gram-negative and Gram-positive bacteria and other species-specific variations (e.g. the flagellar filament protein, Flagellin). We further focus on the mechanism by which the two nucleotide-binding proteins FlhF and FlhG ensure the correct reproduction of flagella place and number (the flagellation pattern). We will finish the review with an overview of current biotechnological applications, and a perspective of how understanding flagella can contribute to developing modules for synthetic approaches.

Keywords: ATPase; GTPase; biotechnology; evolution; flagellum; macromolecular assembly; synthetic.

Publication types

  • Review

MeSH terms

  • Bacteria / ultrastructure*
  • Bacterial Physiological Phenomena*
  • Carrier Proteins / metabolism*
  • Directed Molecular Evolution / methods*
  • Evolution, Molecular*
  • Flagella / diagnostic imaging*
  • Flagella / physiology*
  • Models, Biological
  • Synthetic Biology / methods
  • Ultrasonography

Substances

  • Carrier Proteins