Fast and accurate identification of dermatophytes by matrix-assisted laser desorption ionization-time of flight mass spectrometry: validation in the clinical laboratory

A Packeu; A De Bel; C l'Ollivier; S Ranque; M Detandt; M Hendrickx

doi:10.1128/JCM.01428-14

Fast and accurate identification of dermatophytes by matrix-assisted laser desorption ionization-time of flight mass spectrometry: validation in the clinical laboratory

J Clin Microbiol. 2014 Sep;52(9):3440-3. doi: 10.1128/JCM.01428-14. Epub 2014 Jul 16.

Authors

A Packeu¹, A De Bel², C l'Ollivier³, S Ranque³, M Detandt⁴, M Hendrickx⁵

Affiliations

¹ Scientific Institute of Public Health, Service of Mycology and Aerobiology, Brussels, Belgium [email protected].
² Department of Microbiology and Infection Control, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel (VUB), Brussels, Belgium.
³ Aix Marseille Université, IP-TPT UMR MD3, Marseille, France Parasitology and Mycology Laboratory, CHU Timone-adultes, APHM, Marseille, France.
⁴ Scientific Institute of Public Health, Service of Mycology and Aerobiology, Brussels, Belgium.
⁵ Scientific Institute of Public Health, Service of Mycology and Aerobiology, Brussels, Belgium BCCM/IHEM Fungal Collection, Scientific Institute of Public Health, Mycology and Aerobiology Section, Brussels, Belgium.

Abstract

The performance of a matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) workflow using an extensive reference database for dermatophyte identification was evaluated on 176 clinical strains. Using a direct-deposit procedure after 3 incubation days yielded 40% correct identification. Both increasing incubation time and using an extraction procedure resulted in 100% correct identification.

Publication types

Validation Study

MeSH terms

Arthrodermataceae / chemistry
Arthrodermataceae / classification*
Arthrodermataceae / isolation & purification*
Clinical Laboratory Techniques / methods*
Dermatomycoses / diagnosis*
Dermatomycoses / microbiology*
Humans
Specimen Handling / methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Time Factors