Multiple FLC haplotypes defined by independent cis-regulatory variation underpin life history diversity in Arabidopsis thaliana

Genes Dev. 2014 Aug 1;28(15):1635-40. doi: 10.1101/gad.245993.114. Epub 2014 Jul 17.

Abstract

Relating molecular variation to phenotypic diversity is a central goal in evolutionary biology. In Arabidopsis thaliana, FLOWERING LOCUS C (FLC) is a major determinant of variation in vernalization--the acceleration of flowering by prolonged cold. Here, through analysis of 1307 A. thaliana accessions, we identify five predominant FLC haplotypes defined by noncoding sequence variation. Genetic and transgenic experiments show that they are functionally distinct, varying in FLC expression level and rate of epigenetic silencing. Allelic heterogeneity at this single locus accounts for a large proportion of natural variation in vernalization that contributes to adaptation of A. thaliana.

Keywords: FLOWERING LOCUS C; adaptation; allelic heterogeneity; noncoding polymorphism; vernalization.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adaptation, Physiological / genetics
  • Arabidopsis / genetics*
  • Arabidopsis Proteins / genetics*
  • Epigenesis, Genetic / genetics
  • Gene Expression Regulation, Plant*
  • Gene Silencing
  • Genetic Variation*
  • Haplotypes
  • MADS Domain Proteins / genetics*
  • Plants, Genetically Modified
  • Polymorphism, Single Nucleotide / genetics

Substances

  • Arabidopsis Proteins
  • FLF protein, Arabidopsis
  • MADS Domain Proteins