Identification of SH3 domain proteins interacting with the cytoplasmic tail of the a disintegrin and metalloprotease 10 (ADAM10)

PLoS One. 2014 Jul 18;9(7):e102899. doi: 10.1371/journal.pone.0102899. eCollection 2014.

Abstract

The a disintegrin and metalloproteases (ADAMs) play a pivotal role in the control of development, adhesion, migration, inflammation and cancer. Although numerous substrates of ADAM10 have been identified, the regulation of its surface expression and proteolytic activity is still poorly defined. One current hypothesis is that both processes are in part modulated by protein-protein interactions mediated by the intracellular portion of the protease. For related proteases, especially proline-rich regions serving as docking sites for Src homology domain 3 (SH3) domain-containing proteins proved to be important for mediating regulatory interactions. In order to identify ADAM10-binding SH3 domain proteins, we screened the All SH3 Domain Phager library comprising 305 human SH3 domains using a GST fusion protein with the intracellular region of human ADAM10 as a bait for selection. Of a total of 291 analyzed phage clones, we found 38 SH3 domains that were precipitated with the ADAM10-derived fusion protein but not with GST. We verified the binding to the cytosolic portion of ADAM10 for several candidates by co-immunoprecipitation and/or pull down analyses. Intriguingly, several of the identified proteins have been implicated in regulating surface appearance and/or proteolytic activity of related ADAMs. Thus, it seems likely that they also play a role in ADAM10 biology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ADAM Proteins / metabolism*
  • ADAM10 Protein
  • Amyloid Precursor Protein Secretases / metabolism*
  • Cell Line
  • Cell Line, Tumor
  • Cytoplasm / metabolism*
  • Disintegrins / metabolism*
  • HEK293 Cells
  • Humans
  • Immunoprecipitation / methods
  • Membrane Proteins / metabolism*
  • Protein Binding / physiology
  • Protein Interaction Maps / physiology*
  • Signal Transduction / physiology
  • src Homology Domains / physiology*

Substances

  • Disintegrins
  • Membrane Proteins
  • Amyloid Precursor Protein Secretases
  • ADAM Proteins
  • ADAM10 Protein
  • ADAM10 protein, human

Grants and funding

Funding for this study was provided by the German Research Foundation (DFG), www.dfg.de, DFG CRC 877 project B4 - OJ; DFG CRC 877 project A7 - DK; Individual funding DFG LE2571/3-1 - ML. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.