Characterization and expression pattern of a novel β-defensin in common carp (Cyprinus carpio L.): implications for its role in mucosal immunity

Biosci Biotechnol Biochem. 2014;78(3):430-7. doi: 10.1080/09168451.2014.885830. Epub 2014 Apr 16.

Abstract

β-defensins are a group of cysteine-rich cationic antimicrobial peptides that play antibacterial and antiviral roles in immune systems of vertebrates. Here, we report the cloning and identification of a β-defensin 3 cDNA sequence from the common carp (Cyprinus carpio L.). Sequence alignment and phylogenetic analysis indicated that this β-defensin 3 belonged to the BD-2 group of fish. Real-time PCR showed that the β-defensin 3 mRNA was expressed in all the tissues of normal common carp that we examined and was highly expressed in the spleen and gills. When challenged with Vibrio anguillarum, the expression level of common carp β-defensin 3 mRNA was quickly upregulated in various tissues. Our results indicate that the β-defensin 3 showed markedly high constitutive expression in the gills, and significantly upregulated expression in the hindgut of the common carp after infection, suggesting it plays an important role in the innate and mucosal immunity of common carp.

Keywords: common carp (Cyprinus carpio L.); innate immunity; mRNA expression; molecular cloning; β-defensin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carps / genetics*
  • Carps / immunology
  • Cloning, Molecular
  • Gene Expression Regulation / genetics
  • Gene Expression Regulation / immunology
  • Immunity, Mucosal / genetics*
  • Phylogeny*
  • RNA, Messenger / biosynthesis
  • Sequence Alignment
  • beta-Defensins / biosynthesis*
  • beta-Defensins / genetics

Substances

  • RNA, Messenger
  • beta-Defensins