Ellagic acid protects Lipopolysaccharide/D-galactosamine-induced acute hepatic injury in mice

Lei Gu; Wen-sheng Deng; Ye Liu; Chun-hui Jiang; Long-ci Sun; Xiao-fei Sun; Qing Xu; Hong Zhou

doi:10.1016/j.intimp.2014.07.005

Ellagic acid protects Lipopolysaccharide/D-galactosamine-induced acute hepatic injury in mice

Int Immunopharmacol. 2014 Oct;22(2):341-5. doi: 10.1016/j.intimp.2014.07.005. Epub 2014 Jul 16.

Authors

Lei Gu¹, Wen-sheng Deng¹, Ye Liu¹, Chun-hui Jiang¹, Long-ci Sun¹, Xiao-fei Sun¹, Qing Xu¹, Hong Zhou²

Affiliations

¹ Department of Gastroenterology Surgery, Renji Hospital School of Medicine Shanghai Jiao Tong University, Shanghai 200127, China.
² Department of Gastroenterology Surgery, Renji Hospital School of Medicine Shanghai Jiao Tong University, Shanghai 200127, China. Electronic address: [email protected].

PMID: 25038320
DOI: 10.1016/j.intimp.2014.07.005

Abstract

Ellagic acid, a natural polyphenol found in certain fruits, nuts and vegetables, has been reported to have anti-inflammatory, anti-tumor, and antioxidant activities. However, the effects of ellagic acid on acute hepatic injury have not been reported. In the present study, we investigated the effects of ellagic acid on Lipopolysaccharide/d-galactosamine-induced acute hepatic injury in mice. The results showed that LPS/GalN increased hepatic malondialdehyde (MDA) content, TNF-α level, serum ALT and AST levels and TNF-α level. However, these changes were attenuated by ellagic acid. Western blot analysis showed that ellagic acid inhibited LPS/GalN-induced NF-κB activation. Furthermore, ellagic acid induced the expression of Nrf2 and heme oxygenase-1. In conclusion, our results showed that ellagic acid protected against LPS/GalN-induced liver injury by enhancing the antioxidative defense system and reducing inflammatory response.

Keywords: Ellagic acid; Lipopolysaccharide (LPS); NF-κB; Nrf2.

MeSH terms

Alanine Transaminase / blood
Animals
Anti-Inflammatory Agents / pharmacology
Anti-Inflammatory Agents / therapeutic use*
Aspartate Aminotransferases / blood
Ellagic Acid / pharmacology
Ellagic Acid / therapeutic use*
Galactosamine
Heme Oxygenase-1 / metabolism
Lipopolysaccharides
Liver / drug effects
Liver / metabolism
Liver / pathology
Liver Failure, Acute / blood
Liver Failure, Acute / chemically induced
Liver Failure, Acute / drug therapy*
Liver Failure, Acute / metabolism
Male
Membrane Proteins / metabolism
Mice
Mice, Inbred BALB C
NF-E2-Related Factor 2 / metabolism
NF-kappa B / metabolism
Protective Agents / pharmacology
Protective Agents / therapeutic use*
Tumor Necrosis Factor-alpha / blood
Tumor Necrosis Factor-alpha / metabolism

Substances

Anti-Inflammatory Agents
Lipopolysaccharides
Membrane Proteins
NF-E2-Related Factor 2
NF-kappa B
Nfe2l2 protein, mouse
Protective Agents
Tumor Necrosis Factor-alpha
Ellagic Acid
Galactosamine
Heme Oxygenase-1
Hmox1 protein, mouse
Aspartate Aminotransferases
Alanine Transaminase