An in silico model to predict the potential of dietary proteins as sources of dipeptidyl peptidase IV (DPP-IV) inhibitory peptides

Food Chem. 2014 Dec 15:165:489-98. doi: 10.1016/j.foodchem.2014.05.090. Epub 2014 Jun 2.

Abstract

An in silico approach was developed to predict the potential of 72 dietary proteins to act as a source of dipeptidyl peptidase IV (DPP-IV) inhibitory peptides. The model takes 68 DPP-IV inhibitory peptides (having an IC50 value <2000 μM) and the specific contribution of their amino acids into account. Bovine α-lactalbumin (α-La) and κ-casein (CN) displayed the highest protein coverage (PC, 43.9%) and potency index (PI, 17.9 10(-6) μM(-1)g(-1)), respectively for DPP-IV inhibitory peptides. Sequence alignment of 39 DPP-IV inhibitory peptides having IC50's<200 μM revealed the frequent occurrence of Trp at the N-terminus and Pro at position 2. Canola, chicken egg, oat and wheat were identified as potential sources of DPP-IV inhibitory peptides. In silico approaches may assist in the selection of food proteins for the enzymatic release of DPP-IV inhibitory peptides. The results are relevant to the generation of biofunctional ingredients for glycaemic management.

Keywords: Bioactive peptides; Dipeptidyl peptidase IV inhibitors; Food proteins; In silico model; Type 2 diabetes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caseins / chemistry*
  • Cattle
  • Computer Simulation
  • Dipeptidyl Peptidase 4 / chemistry*
  • Dipeptidyl-Peptidase IV Inhibitors / chemistry*
  • Dipeptidyl-Peptidase IV Inhibitors / isolation & purification
  • Lactalbumin / chemistry*
  • Peptides / chemistry*
  • Peptides / isolation & purification

Substances

  • Caseins
  • Dipeptidyl-Peptidase IV Inhibitors
  • Peptides
  • Lactalbumin
  • Dipeptidyl Peptidase 4