In vitro and in vivo characterization of a high-purity, solvent/detergent-treated factor VIII concentrate: evidence for its therapeutic efficacy in von Willebrand's disease

Eur J Haematol. 1989 Jul;43(1):7-14. doi: 10.1111/j.1600-0609.1989.tb01244.x.

Abstract

A factor VIII (FVIII) concentrate, virus-inactivated by the solvent/detergent procedure, was studied in vitro. In contrast with most high-purity, virus-inactivated FVIII concentrates, it contains not only high levels of von Willebrand factor (vWF) antigen and ristocetin cofactor activity but also high molecular weight forms of von Willebrand factor. Furthermore, it is able to promote platelet adhesion on collagen in a perfusion system. In vivo studies performed in patients with different types of von Willebrand's disease provided evidence that this concentrate corrects Duke's bleeding time and prevents or stops haemorrhages. Thus, the particular advantages of this FVIII/vWF preparation are safety, low content of contamination proteins, and efficacy in von Willebrand's disease.

MeSH terms

  • Antigens / analysis
  • Bleeding Time
  • Detergents
  • Drug Contamination / prevention & control*
  • Factor VIII / isolation & purification
  • Factor VIII / pharmacology
  • Factor VIII / therapeutic use*
  • Humans
  • Molecular Weight
  • Platelet Adhesiveness / drug effects
  • Ristocetin / analysis
  • Ristocetin / blood
  • Solvents
  • Viruses
  • von Willebrand Diseases / blood
  • von Willebrand Diseases / drug therapy*
  • von Willebrand Factor / analysis
  • von Willebrand Factor / immunology

Substances

  • Antigens
  • Detergents
  • Solvents
  • Von Willebrand antigen
  • von Willebrand Factor
  • Ristocetin
  • Factor VIII