[VNTR-genotyping of Vibrio cholerae strains isolated from objects in the territory of Russian Federation in 2012]

Zh Mikrobiol Epidemiol Immunobiol. 2014 Mar-Apr:(2):46-51.
[Article in Russian]

Abstract

Aim: VNTR-typing of Vibrio cholerae strains isolated in the territory of Russian Federation in 2012.

Materials and methods: 71 Vibrio cholerae O3 and 3 V cholerae O1/O139 strains were used in the study. Genotyping was performed by using PCR for 5 VNTR-loci.

Results: Multilocus VNTR-typing allowed to group the strains into 31 VNTR-genotypes. Genotypes were divided among 10 discrete clusters by results of a cluster analysis. The presence of tcpA gene is clearly linked with the presence of VcB locus. Each geographic region was characterized by their own VNTR-genotypes.

Conclusion: In the course of the carried out VNTR-genotyping of V. cholerae isolated in 2012, 2 types of vibrio population formation were detected. A geographic attachment to specific regions was characteristic for most of the genotypes.

MeSH terms

  • Cholera / epidemiology
  • Cholera / microbiology
  • Culture Media
  • Fimbriae Proteins / classification
  • Fimbriae Proteins / genetics*
  • Gene Expression
  • Genetic Loci
  • Genotype
  • Humans
  • Minisatellite Repeats*
  • Multilocus Sequence Typing / methods
  • Phylogeny*
  • Phylogeography
  • Polymerase Chain Reaction
  • Russia / epidemiology
  • Vibrio cholerae / classification
  • Vibrio cholerae / genetics*
  • Vibrio cholerae / isolation & purification

Substances

  • Culture Media
  • TcpA protein, Vibrio cholerae
  • Fimbriae Proteins