Pertussis toxin treatment counteracts the cardiovascular effects of neuropeptide Y and clonidine in the awake unrestrained rat

Neurosci Lett. 1989 Jul 3;101(3):337-41. doi: 10.1016/0304-3940(89)90556-9.

Abstract

The role of G-proteins in the mediation of the cardiovascular effects of neuropeptide Y and the alpha 2-adrenoceptor agonist clonidine was investigated by injections of pertussis toxin (10 micrograms/30 microliters, i.v.t., 24 h) in the awake unrestrained male rat. Treatment with pertussis toxin was found to inhibit the hypotensive and bradycardic actions of neuropeptide Y (1250 pmol) and the hypotensive actions of clonidine (1875 pmol). Control experiments showed that treatment with pertussis toxin caused an approximately 50% reduction in the back-ADP-ribosylation of GTP-binding proteins. These results suggest that G-proteins mediate the central cardiovascular actions of neuropeptide Y and clonidine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cardiovascular Physiological Phenomena*
  • Cardiovascular System / drug effects
  • Cardiovascular System / metabolism
  • Clonidine / pharmacology*
  • GTP-Binding Proteins / metabolism
  • GTP-Binding Proteins / physiology*
  • Male
  • Medulla Oblongata / drug effects
  • Medulla Oblongata / metabolism
  • Medulla Oblongata / physiology*
  • Neuropeptide Y / pharmacology*
  • Pertussis Toxin*
  • Rats
  • Rats, Inbred Strains
  • Virulence Factors, Bordetella / pharmacology*

Substances

  • Neuropeptide Y
  • Virulence Factors, Bordetella
  • Pertussis Toxin
  • GTP-Binding Proteins
  • Clonidine