Effect of handling of plasma samples on histamine radio-enzyme assay

R J Harvima; I T Harvima; E O Kajander; I M Penttilä; H Neittaanmäki; J E Fräki

doi:10.1016/0020-711x(89)90389-3

Effect of handling of plasma samples on histamine radio-enzyme assay

Int J Biochem. 1989;21(6):675-8. doi: 10.1016/0020-711x(89)90389-3.

Authors

R J Harvima¹, I T Harvima, E O Kajander, I M Penttilä, H Neittaanmäki, J E Fräki

Affiliation

¹ Department of Dermatology, University of Kuopio, Finland.

PMID: 2507372
DOI: 10.1016/0020-711x(89)90389-3

Abstract

1. Freeze-thawing of plasma samples increased the histamine level at physiological histamine concentrations as analyzed by radio-enzyme assay, but not by high performance liquid chromatography. 2. Heating of plasma samples decreased the histamine levels. 3. Enzymatic or non-enzymatic formation of histamine during handling of plasma samples was not detected. 4. Some albumin preparations contain histamine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Amine Oxidase (Copper-Containing)
Electrophoresis, Cellulose Acetate
Freezing
Histamine / blood*
Histidine Decarboxylase
Hot Temperature
Humans
Reference Values
Scintillation Counting* / methods
Specimen Handling

Substances

Histamine
Amine Oxidase (Copper-Containing)
Histidine Decarboxylase