The β-Lacta test for direct detection of extended-spectrum-β-lactamase-producing Enterobacteriaceae in urine

Salah Gallah; Dominique Decré; Nathalie Genel; Guillaume Arlet

doi:10.1128/JCM.01629-14

The β-Lacta test for direct detection of extended-spectrum-β-lactamase-producing Enterobacteriaceae in urine

J Clin Microbiol. 2014 Oct;52(10):3792-4. doi: 10.1128/JCM.01629-14. Epub 2014 Jul 30.

Authors

Salah Gallah¹, Dominique Decré², Nathalie Genel³, Guillaume Arlet⁴

Affiliations

¹ Assistance Publique-Hôpitaux de Paris, HUEP, Hôpital Tenon, Department of Bacteriology-Hygiene, Paris, France.
² Université Pierre et Marie Curie-Paris 6, Medical School, Department of Bacteriology, Paris, France Assistance Publique-Hôpitaux de Paris, HUEP, Hôpital Saint Antoine, Department of Bacteriology, Paris, France.
³ Université Pierre et Marie Curie-Paris 6, Medical School, Department of Bacteriology, Paris, France.
⁴ Assistance Publique-Hôpitaux de Paris, HUEP, Hôpital Tenon, Department of Bacteriology-Hygiene, Paris, France Université Pierre et Marie Curie-Paris 6, Medical School, Department of Bacteriology, Paris, France Assistance Publique-Hôpitaux de Paris, HUEP, Hôpital Saint Antoine, Department of Bacteriology, Paris, France [email protected].

Abstract

With the β-Lacta test, production of extended-spectrum β-lactamases (ESBLs) was assayed in 200 urine samples showing Gram-negative bacilli during direct microscopic examination. While 168 samples tested negative, all samples yielding ESBL-producing Enterobacteriaceae after culture gave positive (n = 30) or uninterpretable (n = 2) results. The sensitivity and specificity of ESBL detection were 94% and 100%, respectively.

MeSH terms

Bacteriological Techniques / methods*
Enterobacteriaceae / enzymology*
Enterobacteriaceae / isolation & purification*
Humans
Sensitivity and Specificity
Urine / microbiology*
beta-Lactamases / analysis*

Substances

beta-Lactamases