Association of genetic variants of vit D binding protein (DBP/GC) and of the enzyme catalyzing its 25-hydroxylation (DCYP2R1) and serum vit D in postmenopausal women

Hormones (Athens). 2014 Jul-Sep;13(3):345-52. doi: 10.14310/horm.2002.1484.

Abstract

Objective: To determine if GC (group-specific component globulin) and CYP2R1 genetic variants have an association with serum 25-OHD3 levels, BMD or bone turnover markers in a population of Chinese postmenopausal women.

Design: We randomly selected 1494 postmenopausal women of the Han ethnic group from seven communities in Beijing. BMD was determined by dual energy X-ray absorptiometry; serum bone turnover markers and 25-OHD3 were measured by the automated Roche electrochemiluminescence system; genotypes of GC and CYP2R1 were detected by the TaqMan allelic discrimination assay. Multiple statistic methods were used to test the associations of SNP genotypes and vitamin D levels.

Results: In our sample, 89.6% women had vitamin D deficiency and another 9.8% had vitamin D insufficiency. The variants of rs2298849 (β=0.105, P<0.001) in GC were significantly associated with serum 25-OHD3 levels. Allele G of rs2298849 might be protective for serum 25-OHD3 level. Among the haplotypes of rs222020-rs2298849, CG (β=0.104, P=0.001) corresponded to increasing serum 25-OHD3 concentrations. CYP2R1 polymorphisms showed some significant association with serum β-CTX and P1NP levels.

Conclusions: We found that GC variants had a significant association with serum 25-OHD3 levels among postmenopausal women of the Han ethnic group in Beijing, while CYP2R1 variants were not found to be significant.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Absorptiometry, Photon
  • Aged
  • Asian People / genetics
  • Biomarkers / blood
  • Bone Density / genetics
  • Calcifediol / blood*
  • China
  • Cholestanetriol 26-Monooxygenase / genetics*
  • Collagen Type I / blood
  • Cytochrome P450 Family 2
  • Female
  • Gene Frequency
  • Genetic Association Studies
  • Haplotypes
  • Humans
  • Middle Aged
  • Peptide Fragments / blood
  • Peptides / blood
  • Phenotype
  • Polymorphism, Single Nucleotide*
  • Postmenopause / blood*
  • Postmenopause / ethnology
  • Postmenopause / genetics*
  • Predictive Value of Tests
  • Procollagen / blood
  • Risk Factors
  • Seasons
  • Time Factors
  • Vitamin D Deficiency / blood*
  • Vitamin D Deficiency / diagnosis
  • Vitamin D Deficiency / ethnology
  • Vitamin D Deficiency / genetics*
  • Vitamin D-Binding Protein / genetics*

Substances

  • Biomarkers
  • Collagen Type I
  • Peptide Fragments
  • Peptides
  • Procollagen
  • Vitamin D-Binding Protein
  • collagen type I trimeric cross-linked peptide
  • procollagen Type I N-terminal peptide
  • Cytochrome P450 Family 2
  • CYP2R1 protein, human
  • Cholestanetriol 26-Monooxygenase
  • Calcifediol