Double GC:GC mismatch in dsDNA enhances local dynamics retaining the DNA footprint: a high-resolution NMR study

Anirban Ghosh; Rajiv Kumar Kar; Janarthanan Krishnamoorthy; Subhrangsu Chatterjee; Anirban Bhunia

doi:10.1002/cmdc.201402238

Double GC:GC mismatch in dsDNA enhances local dynamics retaining the DNA footprint: a high-resolution NMR study

ChemMedChem. 2014 Sep;9(9):2059-64. doi: 10.1002/cmdc.201402238. Epub 2014 Jul 30.

Authors

Anirban Ghosh¹, Rajiv Kumar Kar, Janarthanan Krishnamoorthy, Subhrangsu Chatterjee, Anirban Bhunia

Affiliation

¹ Department of Biophysics, Bose Institute, P-1/12 CIT Scheme VII (M), Kolkata 700054 (India).

PMID: 25080019
DOI: 10.1002/cmdc.201402238

Abstract

Mutations in the genome are responsible for several fatal genetic disorders. The default DNA repair mechanism restores the malfunction of the gene caused by mutation to maintain functional regularity and sequential integrity of the cell. Here, we have elucidated the NMR structure and the dynamics of GC mismatched DNA (PDB code: 2MJX) and found that the mismatched DNA still retains the typical B-type helical form, but in the process introduces backbone distortion resulting from frame-shifted base pairs.

Keywords: NMR spectroscopy; duplex DNA; dynamics; mismatch DNA; molecular dynamics simulation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Pair Mismatch*
DNA / chemistry*
DNA / genetics
DNA Footprinting
DNA Repair / genetics
Magnetic Resonance Spectroscopy
Models, Molecular
Mutation
Nucleic Acid Conformation
Thermodynamics

Substances

DNA