A short peptide from frog skin accelerates diabetic wound healing

FEBS J. 2014 Oct;281(20):4633-43. doi: 10.1111/febs.12968. Epub 2014 Sep 8.

Abstract

Delayed wound healing will result in the development of chronic wounds in some diseases, such as diabetes. Amphibian skins possess excellent wound-healing ability and represent a resource for prospective wound-healing promoting compounds. A potential wound-healing promoting peptide (CW49; amino acid sequence APFRMGICTTN) was identified from the frog skin of Odorrana grahami. It promotes wound healing in a murine model with a full-thickness dermal wound in both normal and diabetic animals. In addition to its strong angiogenic ability with respect to the upregulation of some angiogenic proteins, CW49 also showed a significant anti-inflammatory effect in diabetic wounds, which was very important for healing chronic wounds. CW49 had little effect on re-epithelialization, resulting in no significant effect on wound closure rate compared to a vehicle control. Altogether, this indicated that CW49 might accelerate diabetic wound healing by promoting angiogenesis and preventing any excessive inflammatory response. Considering its favorable traits as a small peptide that significantly promotes angiogenesis, CW49 might be an excellent candidate or template for the development of a drug for use in the treatment of diabetic wounds.

Keywords: amphibian; angiogenesis; anti-inflammatory; diabetic wounds; wound healing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anura
  • Blotting, Western
  • Diabetes Mellitus, Experimental / drug therapy*
  • Diabetes Mellitus, Experimental / physiopathology
  • Disease Models, Animal*
  • Enzyme-Linked Immunosorbent Assay
  • Immunoenzyme Techniques
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Obese
  • Neovascularization, Physiologic / drug effects*
  • Peptide Fragments / pharmacology*
  • Skin / blood supply
  • Skin / injuries
  • Skin / metabolism*
  • Wound Healing*

Substances

  • Peptide Fragments