Cloning of human gene encoding prostaglandin endoperoxide synthase and primary structure of the enzyme

C Yokoyama; T Tanabe

doi:10.1016/s0006-291x(89)80049-x

Cloning of human gene encoding prostaglandin endoperoxide synthase and primary structure of the enzyme

Biochem Biophys Res Commun. 1989 Dec 15;165(2):888-94. doi: 10.1016/s0006-291x(89)80049-x.

Authors

C Yokoyama¹, T Tanabe

Affiliation

¹ Department of Pharmacology, National Cardiovascular Center Research Institute, Osaka, Japan.

PMID: 2512924
DOI: 10.1016/s0006-291x(89)80049-x

Abstract

The complete amino acid sequence of human prostaglandin endoperoxide synthase (EC 1.14.99.1, cyclooxygenase) was deduced by cloning and sequence analysis of human genomic DNA coding for the enzyme. The isolated clones covered approximately 40 kilobase pairs of human gene and the protein coding region of the enzyme was distributed into eleven exons, which encoded 599 amino acid residues with a calculated molecular weight of 68,548. Human prostaglandin endoperoxide synthase exhibited 91% amino acid identity with the sheep enzyme.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Base Sequence
Cloning, Molecular*
DNA / genetics
Exons
Genes*
Humans
Molecular Sequence Data
Prostaglandin-Endoperoxide Synthases / genetics*
Restriction Mapping

Substances

DNA
Prostaglandin-Endoperoxide Synthases