Pithomyces species (Montagnulaceae) from clinical specimens: identification and antifungal susceptibility profiles

Med Mycol. 2014 Oct;52(7):748-57. doi: 10.1093/mmy/myu044. Epub 2014 Aug 16.

Abstract

The fungal genus Pithomyces comprises numerous dematiaceous saprobic species commonly found on dead leaves and stems of a great variety of plants. Occasionally, they have been recovered from clinical specimens. We morphologically and molecularly (rDNA sequences) investigated a set of 42 isolates tentatively identified as Pithomyces recovered from clinical specimens in the United States. The predominant species were P. chartarum and P. sacchari (33.3% each), followed by Pithomyces sp. I (28.6%) and P. maydicus (4.8%). Most of the isolates were obtained from samples of superficial tissue (50%), the respiratory tract (21.4%), and the nasal region (19%). In general, these fungi were highly susceptible in vitro to the eight antifungal agents tested.

Keywords: DNA analysis; Pithomyces; antifungal susceptibility; clinical specimens; morphological identification.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antifungal Agents / pharmacology*
  • Ascomycota / classification*
  • Ascomycota / cytology
  • Ascomycota / drug effects*
  • Ascomycota / isolation & purification
  • Cluster Analysis
  • DNA, Fungal / chemistry
  • DNA, Fungal / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Humans
  • Microbial Sensitivity Tests
  • Microscopy
  • Molecular Sequence Data
  • Mycoses / microbiology*
  • Nasal Cavity / microbiology
  • Phylogeny
  • Respiratory System / microbiology
  • Sequence Analysis, DNA
  • Sequence Homology
  • Skin / microbiology
  • United States

Substances

  • Antifungal Agents
  • DNA, Fungal
  • DNA, Ribosomal

Associated data

  • GENBANK/HG326306
  • GENBANK/HG326307
  • GENBANK/HG518059
  • GENBANK/HG518060
  • GENBANK/HG518061
  • GENBANK/HG518062
  • GENBANK/HG518063
  • GENBANK/HG518064
  • GENBANK/HG518065
  • GENBANK/HG518066
  • GENBANK/HG518067
  • GENBANK/HG518068
  • GENBANK/KJ796400
  • GENBANK/KJ796401