Identification of two dominant linear epitopes on the GP3 protein of highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV)

Jia-zeng Chen; Qian Wang; Yun Bai; Bin Wang; Hong-yuan Zhao; Jin-mei Peng; Tong-qing An; Zhi-jun Tian; Guang-zhi Tong

doi:10.1016/j.rvsc.2014.07.011

Identification of two dominant linear epitopes on the GP3 protein of highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV)

Res Vet Sci. 2014 Oct;97(2):238-43. doi: 10.1016/j.rvsc.2014.07.011. Epub 2014 Aug 5.

Authors

Jia-zeng Chen¹, Qian Wang², Yun Bai², Bin Wang², Hong-yuan Zhao², Jin-mei Peng², Tong-qing An², Zhi-jun Tian³, Guang-zhi Tong⁴

Affiliations

¹ College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
² Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
³ Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. Electronic address: [email protected].
⁴ College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China. Electronic address: [email protected].

PMID: 25135493
DOI: 10.1016/j.rvsc.2014.07.011

Abstract

Glycosylated protein 3 (GP3) of PRRSV is variable between different PRRSV strains, so it is helpful for subtype classifying by using distinct epitopes. In this study, two dominant linear GP3 epitopes that were recognized by highly dilute serum in an enzyme-linked immunosorbent assay (ELISA) were identified. Sequence alignments of 36 North American (NA) PRRSV isolates revealed that the epitope H(87)DELGFMV(94) is well conserved, whereas the epitope T(59)RQAAAEILE(68) differs in other low-virulence NA-type strains, which have at least one amino acid mutation in this region. A mutational analysis revealed that none of these mutations could be recognized by the purified antibodies directed against the corresponding epitope, indicating that the genetic variations altered the antigenicity of the antigenic region. Using ELISA, we also found that antibodies directed against the two epitopes were present in more than 45 of 50 HP-PRRS-positive pig sera, suggesting that their antigenicity is excellent in vivo.

Keywords: Antibody purification; Epitope; GP3; HP-PRRSV.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Antibodies, Viral / blood
Antibodies, Viral / immunology
Antigens, Viral / immunology
Enzyme-Linked Immunosorbent Assay
Epitopes / genetics*
Epitopes / immunology*
Glycosylation
Molecular Sequence Data
Mutation / genetics
Porcine Reproductive and Respiratory Syndrome / blood
Porcine Reproductive and Respiratory Syndrome / immunology*
Porcine respiratory and reproductive syndrome virus / genetics*
Porcine respiratory and reproductive syndrome virus / immunology*
Sequence Alignment
Swine
Viral Proteins / genetics*
Viral Proteins / immunology*
Viral Proteins / metabolism

Substances

Antibodies, Viral
Antigens, Viral
Epitopes
Viral Proteins