Spherical nucleic acids as a divergent platform for synthesizing RNA-nanoparticle conjugates through enzymatic ligation

ACS Nano. 2014 Sep 23;8(9):8837-43. doi: 10.1021/nn503601s. Epub 2014 Aug 21.

Abstract

Herein, we describe a rapid, divergent method for using spherical nucleic acids (SNAs) as a universal platform for attaching RNA to DNA-modified nanoparticles using enzyme-mediated techniques. This approach provides a sequence-specific method for the covalent attachment of one or more in vitro transcribed RNAs to a universal SNA scaffold, regardless of RNA sequence. The RNA-nanoparticle constructs are shown to effectively knock down two different gene targets using a single, dual-ligated nanoparticle construct.

Keywords: T4 DNA ligase; enzymatic ligation; gene knockdown; nanoparticle; siRNA.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Base Sequence
  • DNA Ligases / metabolism*
  • HeLa Cells
  • Humans
  • Models, Molecular
  • Nanoparticles / chemistry*
  • Nanotechnology / methods*
  • Nucleic Acid Conformation*
  • Nucleic Acid Hybridization
  • RNA / chemistry*
  • RNA / genetics

Substances

  • RNA
  • DNA Ligases