Polymorphism of the IL-8 gene and the risk of ovarian cancer

D Koensgen; D Bruennert; S Ungureanu; D Sofroni; E I Braicu; J Sehouli; A Sümnig; S Delogu; M Zygmunt; P Goyal; M Evert; S Olek; K E Biebler; A Mustea

doi:10.1016/j.cyto.2014.07.254

Polymorphism of the IL-8 gene and the risk of ovarian cancer

Cytokine. 2015 Feb;71(2):334-8. doi: 10.1016/j.cyto.2014.07.254. Epub 2014 Aug 21.

Authors

D Koensgen¹, D Bruennert², S Ungureanu³, D Sofroni⁴, E I Braicu⁵, J Sehouli⁶, A Sümnig⁷, S Delogu⁸, M Zygmunt⁹, P Goyal¹⁰, M Evert¹¹, S Olek¹², K E Biebler¹³, A Mustea¹⁴

Affiliations

¹ Department of Gynecology and Obstetrics, Medical University Greifswald, Ferdinand-Sauerbruch-Str.,17475 Greifswald, Germany. Electronic address: [email protected].
² Department of Gynecology and Obstetrics, Medical University Greifswald, Ferdinand-Sauerbruch-Str.,17475 Greifswald, Germany. Electronic address: [email protected].
³ Department of Gynecology and Obstetrics, Medical University Greifswald, Ferdinand-Sauerbruch-Str.,17475 Greifswald, Germany; Department of Gynecology, Oncological Institute, Testemitanu Str. 30, 2025 Chisinau, Moldavia. Electronic address: [email protected].
⁴ Department of Gynecology, Oncological Institute, Testemitanu Str. 30, 2025 Chisinau, Moldavia. Electronic address: [email protected].
⁵ Department of Gynecology, European Competence Center for Ovarian Cancer, Campus Virchow Klinikum, Charité - Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany. Electronic address: [email protected].
⁶ Department of Gynecology, European Competence Center for Ovarian Cancer, Campus Virchow Klinikum, Charité - Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany. Electronic address: [email protected].
⁷ Institute for Immunology and Transfusion Medicine, Ferdinand-Sauerbruch-Str., 17475 Greifswald, Germany. Electronic address: [email protected].
⁸ Department of Gynecology and Obstetrics, Medical University Greifswald, Ferdinand-Sauerbruch-Str.,17475 Greifswald, Germany. Electronic address: [email protected].
⁹ Department of Gynecology and Obstetrics, Medical University Greifswald, Ferdinand-Sauerbruch-Str.,17475 Greifswald, Germany. Electronic address: [email protected].
¹⁰ Department of Gynecology and Obstetrics, Medical University Greifswald, Ferdinand-Sauerbruch-Str.,17475 Greifswald, Germany. Electronic address: [email protected].
¹¹ Institute for Pathology, Medical University Greifswald, Friedrich-Loeffler-Str. 23e, 17487 Greifswald, Germany. Electronic address: [email protected].
¹² Epiontis GmbH, Rudower Chaussee 29, 12489 Berlin, Germany. Electronic address: [email protected].
¹³ Institute for Biometrics and Medical Informatics, Walther-Rathenau-Straße 48, 17475 Greifswald, Germany. Electronic address: [email protected].
¹⁴ Department of Gynecology and Obstetrics, Medical University Greifswald, Ferdinand-Sauerbruch-Str.,17475 Greifswald, Germany. Electronic address: [email protected].

PMID: 25151495
DOI: 10.1016/j.cyto.2014.07.254

Abstract

Ovarian cancer still represents a challenge in gynecological oncology. Most patients are diagnosed in an advanced tumor stage. No specific screening or prevention strategies for ovarian cancer exist as of yet. Interleukin 8 (IL-8) is a pro-inflammatory chemokine known for its angiogenetic activity, and is supposedly responsible for tumor-associated angiogenesis in several malignant tumors. The aim of the study was to investigate the susceptibility of patients with an IL-8 gene polymorphism to developing ovarian cancer. Four single nucleotide polymorphisms (SNPs) (IL-8 -251, IL-8 +781, IL-8 +1633 and IL-8 +2767) of the IL-8 gene were screened, using the PCR method in 268 patients with ovarian cancer and 426 healthy women as a control group. Significant associations were noted in patients with the IL-8 +781 (T/T) genotype (p=0.0048) with increased frequencies of ovarian cancer, while women with the IL-8 +781 (C/C) allele suffer from ovarian cancer significantly less frequently (p=0.0003). Furthermore, the IL-8 +2767 (T/T) genotype is also associated with a higher risk of ovarian cancer (p=0.0177). Our results indicate, for the first time, that IL-8 polymorphism is associated with ovarian cancer.

Keywords: IL-8 polymorphism; Ovarian cancer; PCR.

Publication types

Multicenter Study

MeSH terms

Adolescent
Adult
Aged
Aged, 80 and over
Alleles
Base Sequence
Case-Control Studies
Cytokines / metabolism
Europe
Female
Genetic Predisposition to Disease
Genotype
Humans
Interleukin-8 / genetics*
Middle Aged
Molecular Sequence Data
Neovascularization, Pathologic
Ovarian Neoplasms / genetics*
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Polymorphism, Single Nucleotide*
Prospective Studies
Young Adult

Substances

CXCL8 protein, human
Cytokines
Interleukin-8