Tau overexpression impacts a neuroinflammation gene expression network perturbed in Alzheimer's disease

PLoS One. 2014 Aug 25;9(8):e106050. doi: 10.1371/journal.pone.0106050. eCollection 2014.

Abstract

Filamentous inclusions of the microtubule-associated protein, tau, define a variety of neurodegenerative diseases known as tauopathies, including Alzheimer's disease (AD). To better understand the role of tau-mediated effects on pathophysiology and global central nervous system function, we extensively characterized gene expression, pathology and behavior of the rTg4510 mouse model, which overexpresses a mutant form of human tau that causes Frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17). We found that the most predominantly altered gene expression pathways in rTg4510 mice were in inflammatory processes. These results closely matched the causal immune function and microglial gene-regulatory network recently identified in AD. We identified additional gene expression changes by laser microdissecting specific regions of the hippocampus, which highlighted alterations in neuronal network activity. Expression of inflammatory genes and markers of neuronal activity changed as a function of age in rTg4510 mice and coincided with behavioral deficits. Inflammatory changes were tau-dependent, as they were reversed by suppression of the tau transgene. Our results suggest that the alterations in microglial phenotypes that appear to contribute to the pathogenesis of Alzheimer's disease may be driven by tau dysfunction, in addition to the direct effects of beta-amyloid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alzheimer Disease / genetics*
  • Animals
  • Chromosomes, Human, Pair 17 / genetics
  • Disease Models, Animal
  • Female
  • Frontotemporal Dementia / genetics
  • Gene Expression / genetics*
  • Gene Regulatory Networks / genetics*
  • Hippocampus / metabolism
  • Humans
  • Inflammation / genetics*
  • Mice
  • Microglia / metabolism
  • Microtubule-Associated Proteins / genetics
  • Neurodegenerative Diseases / genetics
  • Neurons / metabolism
  • Parkinsonian Disorders / genetics
  • tau Proteins / genetics*

Substances

  • Microtubule-Associated Proteins
  • tau Proteins

Grants and funding

The Bristol-Myers Squibb internal R&D budget provided all sources of funding. The funders had no rolein the study design, data collection and analysis, decision to publish, or preparation of the manuscript.