MRI molecular imaging using GLUT1 antibody-Fe3O4 nanoparticles in the hemangioma animal model for differentiating infantile hemangioma from vascular malformation

Chul-Ho Sohn; Seung Pyo Park; Seung Hong Choi; Sung-Hye Park; Sukwha Kim; Lianji Xu; Sang-Hyon Kim; Ji An Hur; Jaehoon Choi; Tae Hyun Choi

doi:10.1016/j.nano.2014.08.003

MRI molecular imaging using GLUT1 antibody-Fe3O4 nanoparticles in the hemangioma animal model for differentiating infantile hemangioma from vascular malformation

Nanomedicine. 2015 Jan;11(1):127-35. doi: 10.1016/j.nano.2014.08.003. Epub 2014 Aug 25.

Authors

Chul-Ho Sohn¹, Seung Pyo Park², Seung Hong Choi², Sung-Hye Park³, Sukwha Kim⁴, Lianji Xu⁵, Sang-Hyon Kim⁶, Ji An Hur⁷, Jaehoon Choi⁸, Tae Hyun Choi⁹

Affiliations

¹ Department of Radiology, Seoul National University College of Medicine, Seoul, Republic of Korea.
² Center for Nanoparticle Research, Institute for Basic Science, and School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea.
³ Department of Pathology, Seoul National University College of Medicine, Seoul, Republic of Korea.
⁴ Department of Plastic and Reconstructive Surgery, Institute of Human-Environment Interface Biology, Seoul National University College of Medicine, Seoul, Republic of Korea.
⁵ Department of Plastic and Reconstructive Surgery, Beijing Tongren Hospital, Capital Medical University, People's Republic of China.
⁶ Department of Internal Medicine, Keimyung University Dongsan Medical Center, Daegu, Republic of Korea.
⁷ Department of Internal Medicine, School of Medicine, Yeungnam University, Daegu, Republic of Korea.
⁸ Department of Plastic and Reconstructive Surgery, Keimyung University School of Medicine, Daegu, Republic of Korea.
⁹ Department of Plastic and Reconstructive Surgery, Institute of Human-Environment Interface Biology, Seoul National University College of Medicine, Seoul, Republic of Korea. Electronic address: [email protected].

PMID: 25168935
DOI: 10.1016/j.nano.2014.08.003

Abstract

The purpose of this study is to evaluate the efficacy of glucose transporter protein 1 (GLUT1) antibody-conjugated iron oxide nanoparticles (Fe3O4 NPs) as magnetic resonance imaging (MRI) molecular imaging agents for differentiating infantile hemangioma from vascular malformation in the hemangioma animal model. The conjugation of Fe3O4 NPs with anti-GLUT1 antibodies leads to a significantly increased uptake of NPs by human umbilical vein endothelial cells. MRI imaging following the intravenous injection of GLUT1 antibody-Fe3O4 NPs yielded a significantly lower signal intensity than did unconjugated Fe3O4 NPs. Upon histological examination of the GLUT1 antibody-Fe3O4 NPs, Prussian blue-stained NPs were identified in CD31-positive endothelial cells of hemangioma. In contrast, when treated with unconjugated Fe3O4 NPs, Prussian blue-stained NPs were found in macrophages rather than in endothelial cells. GLUT1 antibody conjugation can effectively target the injected Fe3O4 NPs to GLUT1-positive tumor cells in infantile hemangioma.

Keywords: GLUT1; Infantile hemangioma; Iron oxide nanoparticle; MRI.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Coloring Agents / chemistry
Contrast Media / chemistry
Diagnosis, Differential
Female
Ferric Compounds / chemistry
Ferrocyanides / chemistry
Glucose Transporter Type 1 / metabolism*
Hemangioma / diagnosis*
Human Umbilical Vein Endothelial Cells
Humans
Magnetic Resonance Imaging / methods*
Magnetite Nanoparticles / chemistry*
Mice
Mice, Inbred BALB C
Mice, Nude
Models, Animal
Molecular Imaging / methods*
Vascular Malformations / diagnosis*

Substances

Coloring Agents
Contrast Media
Ferric Compounds
Ferrocyanides
Glucose Transporter Type 1
Magnetite Nanoparticles
SLC2A1 protein, human
ferric oxide
ferric ferrocyanide