Direct isolation (without enrichment) of Listeria monocytogenes in milk

L Dominguez; V Briones; M Blanco; J F Fernandez-Garayzabal; J L Blanco; J A Vazquez; G Suarez

Direct isolation (without enrichment) of Listeria monocytogenes in milk

Acta Microbiol Hung. 1989;36(2-3):297-301.

Authors

L Dominguez¹, V Briones, M Blanco, J F Fernandez-Garayzabal, J L Blanco, J A Vazquez, G Suarez

Affiliation

¹ Department of Animal Pathology, Microbiology and Immunity, Veterinary Faculty, Madrid, Spain.

PMID: 2517175

Abstract

In the present work we have developed a direct method (without enrichment) for the isolation of listeriae in milk, using the combinated action of SDS (sodium dodecyl sulphate), centrifugation and selective media. It is possible to identify even 0.5 listeriae/ml amongst 7.2 x 10(7) c.f.u. contaminants/ml.

MeSH terms

Animals
Azides / pharmacology
Bacteriological Techniques*
Centrifugation
Culture Media
Food Microbiology*
Listeria monocytogenes / drug effects
Listeria monocytogenes / isolation & purification*
Milk / microbiology*
Sodium Azide
Sodium Dodecyl Sulfate / pharmacology

Substances

Azides
Culture Media
Sodium Dodecyl Sulfate
Sodium Azide