Toll-like receptor expression in the nervous system of bovine alpha-herpesvirus-infected calves

M S Marin; S Quintana; M R Leunda; A C Odeón; S E Pérez

doi:10.1016/j.rvsc.2014.06.014

Toll-like receptor expression in the nervous system of bovine alpha-herpesvirus-infected calves

Res Vet Sci. 2014 Oct;97(2):422-9. doi: 10.1016/j.rvsc.2014.06.014. Epub 2014 Jul 5.

Authors

M S Marin¹, S Quintana², M R Leunda³, A C Odeón⁴, S E Pérez¹

Affiliations

¹ Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rivadavia 1917, C1033AAJ, Buenos Aires, Argentina.
² Instituto de Análisis Fares Taie, Rivadavia 3331, (7600) Mar del Plata, Buenos Aires, Argentina.
³ Instituto Nacional de Tecnología Agropecuaria (INTA), Estación Experimental Agropecuaria Balcarce, Ruta 226 Km 73.5 (7620), Balcarce, Buenos Aires, Argentina.
⁴ Instituto Nacional de Tecnología Agropecuaria (INTA), Estación Experimental Agropecuaria Balcarce, Ruta 226 Km 73.5 (7620), Balcarce, Buenos Aires, Argentina. Electronic address: [email protected].

PMID: 25172667
DOI: 10.1016/j.rvsc.2014.06.014

Abstract

In this study, the expression levels of viral Toll-like receptors (TLRs) in the nervous system of bovine herpesvirus type 5 (BoHV-5)-infected calves were investigated. A significant increase in the expression of TLRs 3 and 7-9 was found in the anterior cerebral cortex during acute infection and viral reactivation. In the trigeminal ganglia, only TLR9 expression was significantly affected. The magnitude of the increase was lower in BoHV-1-infected calves, suggesting that a restricted immune response might protect against exacerbated inflammatory responses in the brain. This work describes, for the first time, the involvement of TLRs 3 and 7-9 in the recognition of BoHV in the bovine nervous system, indicating that the expression of these receptors might be associated with the development of neurological disease. Modulation of the signalling pathways mediated by TLRs might provide an effective approach to control the neuro-immune response to BoHV-5, which may be responsible for neurological lesions.

Keywords: Bovine herpesviruses; Encephalitis; Expression patterns; Toll-like receptor.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Administration, Intranasal
Animals
Cattle
Cattle Diseases / metabolism*
Cattle Diseases / pathology
Cattle Diseases / virology*
Cerebral Cortex / metabolism
Cerebral Cortex / pathology
Cerebral Cortex / virology
DNA, Viral / metabolism
Encephalitis, Viral / metabolism
Encephalitis, Viral / pathology
Encephalitis, Viral / veterinary*
Herpesviridae Infections / metabolism
Herpesviridae Infections / pathology
Herpesviridae Infections / veterinary*
Herpesvirus 5, Bovine / genetics
Herpesvirus 5, Bovine / isolation & purification
Herpesvirus 5, Bovine / pathogenicity*
Meningoencephalitis / metabolism
Meningoencephalitis / pathology
Meningoencephalitis / veterinary*
Nervous System / metabolism*
Nervous System / pathology
Nervous System / virology
Signal Transduction
Toll-Like Receptor 3 / metabolism
Toll-Like Receptor 7 / metabolism
Toll-Like Receptor 8 / metabolism
Toll-Like Receptor 9 / metabolism
Toll-Like Receptors / metabolism*
Trigeminal Ganglion / metabolism
Trigeminal Ganglion / pathology
Trigeminal Ganglion / virology

Substances

DNA, Viral
Toll-Like Receptor 3
Toll-Like Receptor 7
Toll-Like Receptor 8
Toll-Like Receptor 9
Toll-Like Receptors