Abstract
The exquisite selectivity of chemical reactions enables the study of rare DNA bases. However, chemical modification of the genome can affect downstream analysis. We report a PCR bias caused by such modification, and exemplify a solution with the synthesis and characterization of a cleavable aldehyde-reactive biotinylation probe.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aldehydes / chemistry
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Biotinylation
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DNA / chemistry
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DNA / metabolism*
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DNA Adducts / chemistry
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DNA Probes / chemical synthesis
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DNA Probes / chemistry
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Hydrogen-Ion Concentration
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Oxidation-Reduction
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Real-Time Polymerase Chain Reaction*
Substances
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Aldehydes
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DNA Adducts
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DNA Probes
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DNA