[Resistance caused by hyperproduction of chromosomal beta-lactamase in Pseudomonas aeruginosa]

Enferm Infecc Microbiol Clin. 1989 Dec;7(10):530-4.
[Article in Spanish]

Abstract

From 120 Pseudomonas aeruginosa strains selected for their slight susceptibility to ceftazidime (MIC greater than or equal to 16 micrograms/ml) we studied the characteristics of beta-lactamases and their susceptibility to aminoglycoside and to beta-lactam antibiotics. The quantitative spectrum, chromosomic beta-lactamase hyperproduction and the isoelectric point of beta-lactamases were also studied as well as the MIC in solid medium, inoculum 5 x 10(4) cfu. About 14.7% of strains moderately susceptible to ceftazidime and 88.4% of those resistant, were hyperproducers of chromosomic beta-lactamases. All the strains were resistant to ureidopenicillins, cefotaxime and moxalactam, 55.8% to monobactams and 35% were also resistant to cefsulodine; all of them were susceptible to imipenem. In bacteria isolated from twelve patients a loss of susceptibility could be observed against ceftazidime and other beta-lactams. There was also an increase in chromosomic beta-lactamase production during the treatment with antibiotics.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aminoglycosides
  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / biosynthesis*
  • Bacterial Proteins / isolation & purification
  • Chromosomes, Bacterial
  • Drug Resistance, Microbial
  • Humans
  • Pseudomonas Infections / drug therapy
  • Pseudomonas Infections / microbiology*
  • Pseudomonas aeruginosa / drug effects
  • Pseudomonas aeruginosa / enzymology*
  • Pseudomonas aeruginosa / genetics
  • Pseudomonas aeruginosa / isolation & purification
  • beta-Lactamases / biosynthesis*
  • beta-Lactamases / isolation & purification
  • beta-Lactams

Substances

  • Aminoglycosides
  • Anti-Bacterial Agents
  • Bacterial Proteins
  • beta-Lactams
  • beta-Lactamases