The Effect of Long Term Pre/postnatal Low/high Dose Nicotine Exposure on Tissue Oxidant/antioxidant Status and DNA Damage in Rats

Drug Res (Stuttg). 2015 Aug;65(8):432-6. doi: 10.1055/s-0034-1387739. Epub 2014 Sep 4.

Abstract

Background: Most women do not stop smoking either during pregnancy or in the lactation period. This study was carried out to investigate the effect of long term per oral pre/postnatal low/high dose nicotine exposure on fetal plasma/tissue oxidant-antioxidant status in rats.

Methods: The study groups were composed of pups whose parents used or did not use nicotine in pregnancy and lactation period. The pups were divided into 3 groups, each consisting of 10 rats; the control group (normal drinking water), low and high dose nicotine groups according to the dose of nicotine (0.4 mg/kg and 6.0 mg/kg BW/day, respectively) given per oral in drinking water. At the end of the 12(th) month, tissue/hemolysate/plasma oxidant-antioxidant status parameters and 8-hydroxy-2-deoxy-guanosine levels were measured.

Results: Plasma cotinine levels were higher in nicotine groups compared to controls (p<0.01). A significant increase in liver malonyldialdehyde levels (p<0.05) and a significant decrease in kidney superoxide dismutase activities (p<0.05) were determined in both nicotine groups compared to controls while no statistically significant difference was found in the other parameters.

Conclusion: This investigation showed that long term nicotine exposure during-after pregnancy may have an adverse effect on vital organs of the offspring via impairing tissue oxidant/antioxidant balance. Liver and kidney seem to be the mostly affected organs possibly due to their major roles in nicotine metabolism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / metabolism*
  • DNA Damage / drug effects*
  • Environmental Exposure / adverse effects*
  • Female
  • Fetus / drug effects
  • Nicotine / adverse effects*
  • Oxidants / metabolism*
  • Oxidation-Reduction / drug effects*
  • Postnatal Care
  • Pregnancy
  • Prenatal Exposure Delayed Effects / etiology*
  • Rats

Substances

  • Antioxidants
  • Oxidants
  • Nicotine