Multilocus sequence typing of Mycobacterium xenopi

David C Alexander; Theodore K Marras; Jennifer H Ma; Samia Mirza; Daniel Liu; Julianne V Kus; Hafid Soualhine; Vincent Escuyer; David Warshauer; Sarah K Brode; David J Farrell; Frances B Jamieson

doi:10.1128/JCM.01601-14

Multilocus sequence typing of Mycobacterium xenopi

J Clin Microbiol. 2014 Nov;52(11):3973-7. doi: 10.1128/JCM.01601-14. Epub 2014 Sep 10.

Authors

Affiliations

¹ Public Health Ontario Laboratories, Public Health Ontario, Toronto, ON, Canada Saskatchewan Disease Control Laboratory, Saskatchewan Ministry of Health, Regina, SK, Canada.
² University of Toronto, Department of Medicine, Toronto, ON, Canada University Health Network and Mount Sinai Hospital, Department of Medicine, Toronto, ON, Canada.
³ Public Health Ontario Laboratories, Public Health Ontario, Toronto, ON, Canada.
⁴ Public Health Ontario Laboratories, Public Health Ontario, Toronto, ON, Canada University of Toronto, Department of Laboratory Medicine and Pathobiology, Toronto, ON, Canada.
⁵ Laboratoire de Santé Publique du Québec, Institut National de Santé Publique du Québec, Sainte-Anne-de-Bellevue, QC, Canada.
⁶ Wadsworth Center, New York State Department of Health, Albany, New York, USA.
⁷ Wisconsin State Laboratory of Hygiene, Madison, Wisconsin, USA.
⁸ Public Health Ontario Laboratories, Public Health Ontario, Toronto, ON, Canada University of Toronto, Department of Laboratory Medicine and Pathobiology, Toronto, ON, Canada [email protected].

Abstract

Mycobacterium xenopi is an opportunistic mycobacterial pathogen of increasing clinical importance. Surveillance of M. xenopi is hampered by the absence of tools for genotyping and molecular epidemiology. In this study, we describe the development and evaluation of an effective multilocus sequence typing strategy for M. xenopi.

Publication types

Evaluation Study

MeSH terms

Humans
Molecular Epidemiology / methods
Multilocus Sequence Typing / methods*
Mycobacterium Infections, Nontuberculous / epidemiology
Mycobacterium Infections, Nontuberculous / microbiology*
Mycobacterium xenopi / classification*
Mycobacterium xenopi / genetics*